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rencell nsc maintenance medium  (Merck KGaA)

 
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    Structured Review

    Merck KGaA rencell nsc maintenance medium
    Fluorescence microscopic images <t>ReNcell®CX</t> cell viability after one and 7 days of cultivation (37°C, 5% CO 2 ) in five different GelNB hydrogel compositions . “high DTT:IKVAV” refers to a high crosslinker concentration, “low DTT:IKVAV” to a low crosslinker concentration. Encapsulated cell concentration 1 × 10 6 cells/mL. Green: Calcein-AM staining (viable cells), Red: Propidium iodide staining (dead cells).
    Rencell Nsc Maintenance Medium, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rencell+nsc+maintenance+medium/rencell+nsc+medium/pmc12163324-93-5-12
    Average 90 stars, based on 1 article reviews
    rencell nsc maintenance medium - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization"

    Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2025.1579580

    Fluorescence microscopic images ReNcell®CX cell viability after one and 7 days of cultivation (37°C, 5% CO 2 ) in five different GelNB hydrogel compositions . “high DTT:IKVAV” refers to a high crosslinker concentration, “low DTT:IKVAV” to a low crosslinker concentration. Encapsulated cell concentration 1 × 10 6 cells/mL. Green: Calcein-AM staining (viable cells), Red: Propidium iodide staining (dead cells).
    Figure Legend Snippet: Fluorescence microscopic images ReNcell®CX cell viability after one and 7 days of cultivation (37°C, 5% CO 2 ) in five different GelNB hydrogel compositions . “high DTT:IKVAV” refers to a high crosslinker concentration, “low DTT:IKVAV” to a low crosslinker concentration. Encapsulated cell concentration 1 × 10 6 cells/mL. Green: Calcein-AM staining (viable cells), Red: Propidium iodide staining (dead cells).

    Techniques Used: Fluorescence, Concentration Assay, Staining

    Mean UnaG fluorescence of the ReNcell®CX-HRE-dUnaG hypoxia reporter cells in 2D cell culture after 24 h and 48 h cultivation in different oxygen concentrations (1%, 2%, 5%, 10%, 21%). Mean UnaG fluorescence determined via flow cytometry. Mean n = 3 ± SD; *** p < 0.001.
    Figure Legend Snippet: Mean UnaG fluorescence of the ReNcell®CX-HRE-dUnaG hypoxia reporter cells in 2D cell culture after 24 h and 48 h cultivation in different oxygen concentrations (1%, 2%, 5%, 10%, 21%). Mean UnaG fluorescence determined via flow cytometry. Mean n = 3 ± SD; *** p < 0.001.

    Techniques Used: Fluorescence, Cell Culture, Flow Cytometry

    Visualization of hypoxic response of ReNcell®CX-HRE-dUnaG hypoxia reporter cells using cell concentrations ranging from 1 × 10 6 to 15 × 10 6 cells/mL in GelNB hydrogel composition 2 (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV; 800 Pa) after 1-, 3- and 7-days incubation at 37°C and 21% O 2 .
    Figure Legend Snippet: Visualization of hypoxic response of ReNcell®CX-HRE-dUnaG hypoxia reporter cells using cell concentrations ranging from 1 × 10 6 to 15 × 10 6 cells/mL in GelNB hydrogel composition 2 (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV; 800 Pa) after 1-, 3- and 7-days incubation at 37°C and 21% O 2 .

    Techniques Used: Incubation

    Mean UnaG fluorescence intensity of ReNcell®CX-HRE-dUnaG reporter cells after 1-, 3- and 7-days cultivation in GelNB hydrogel (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV) using cell concentrations ranging from 1 x 10 6 to 15 x 10 6 cells/mL. Flow cytometric measurement after collagenase digestion of hydrogel. Mean n = 3 ± SD; ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Mean UnaG fluorescence intensity of ReNcell®CX-HRE-dUnaG reporter cells after 1-, 3- and 7-days cultivation in GelNB hydrogel (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV) using cell concentrations ranging from 1 x 10 6 to 15 x 10 6 cells/mL. Flow cytometric measurement after collagenase digestion of hydrogel. Mean n = 3 ± SD; ** p < 0.01, *** p < 0.001.

    Techniques Used: Fluorescence

    Related Articles

    Cell Culture:

    Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization
    Article Snippet: .. The cells were cultivated with serum free ReNcell NSC Maintenance Medium (Merck KGaA) supplemented with 20 ng/mL epidermal growth factor (EGF; Thermo Fisher Scientific Inc.), 20 ng/mL basic fibroblast growth factor (bFGF; Bio-Techne) and 50 μg/mL gentamicin (Merck KGaA) in laminin-coated (20 μg/mL (Bio-Techne) in DMEM/F12) cell culture T-flasks. ..

    Derivative Assay:

    Article Title: ARL6IP1 gene delivery reduces neuroinflammation and neurodegenerative pathology in hereditary spastic paraplegia model
    Article Snippet: HEK293, HeLa, U2OS cell lines (KCTC), and GFP-LC3B HeLa (generated GFP-LC3B-expressing cell line) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Welgene) with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and penicillin–streptomycin (P/S, #15140122; Thermo Fisher Scientific) in a humidified incubator with 5% CO 2 at 37°C. .. ReNcell CX cells, derived from the cortical region of human fetal brain tissue (#SCC007; Merck Millipore) as an immortalized human progenitor cell line, were maintained in ReNcell NSC maintenance medium (SCM005; Merck Millipore) supplemented with 20 ng/ml epidermal growth factor (AF-100-15; PeproTech), 20 ng/ml basic fibroblast growth factor (100-18B; PeproTech), and P/S. ..



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    Fluorescence microscopic images <t>ReNcell®CX</t> cell viability after one and 7 days of cultivation (37°C, 5% CO 2 ) in five different GelNB hydrogel compositions . “high DTT:IKVAV” refers to a high crosslinker concentration, “low DTT:IKVAV” to a low crosslinker concentration. Encapsulated cell concentration 1 × 10 6 cells/mL. Green: Calcein-AM staining (viable cells), Red: Propidium iodide staining (dead cells).
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    Image Search Results


    Expression of total BDNF mRNA ( A , BDNF-IX ), the BDNF exon IV containing transcript ( B , BDNF IV-IX, RefSeq: NM_001048141.1), BDNF-DT ( C ), and BDNF-AS-DT readthrough transcript ( D ) is shown at different time-points after KCl-induced depolarization, in human ReNcell VM neural progenitor cells. RNA expression, measured with qPCR, is represented as fold-change vs. vehicle. KCl-induced neuronal depolarization causes an increase of the expression of BDNF , BDNF-DT , and BDNF-AS-DT . RNA expression, measured with qPCR, is represented as fold-change vs. Vehicle. All data are expressed as mean□±□standard error of the mean (SEM). Individual comparisons; *p□<□0.05, **p□<□0.01.

    Journal: medRxiv

    Article Title: BDNF-DT and BDNF-AS-DT : Novel Genes in the BDNF locus

    doi: 10.1101/2025.07.10.25331311

    Figure Lengend Snippet: Expression of total BDNF mRNA ( A , BDNF-IX ), the BDNF exon IV containing transcript ( B , BDNF IV-IX, RefSeq: NM_001048141.1), BDNF-DT ( C ), and BDNF-AS-DT readthrough transcript ( D ) is shown at different time-points after KCl-induced depolarization, in human ReNcell VM neural progenitor cells. RNA expression, measured with qPCR, is represented as fold-change vs. vehicle. KCl-induced neuronal depolarization causes an increase of the expression of BDNF , BDNF-DT , and BDNF-AS-DT . RNA expression, measured with qPCR, is represented as fold-change vs. Vehicle. All data are expressed as mean□±□standard error of the mean (SEM). Individual comparisons; *p□<□0.05, **p□<□0.01.

    Article Snippet: The ReNcell VM line was maintained in ReNCell NSC Maintenance medium (Millipore Sigma), supplemented with Antibiotic-Antimycotic (Gibco), 20 ng/ml EGF (Peprotech) and 20 ng/ml bFGF (Peprotech).

    Techniques: Expressing, RNA Expression

    Fluorescence microscopic images ReNcell®CX cell viability after one and 7 days of cultivation (37°C, 5% CO 2 ) in five different GelNB hydrogel compositions . “high DTT:IKVAV” refers to a high crosslinker concentration, “low DTT:IKVAV” to a low crosslinker concentration. Encapsulated cell concentration 1 × 10 6 cells/mL. Green: Calcein-AM staining (viable cells), Red: Propidium iodide staining (dead cells).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization

    doi: 10.3389/fbioe.2025.1579580

    Figure Lengend Snippet: Fluorescence microscopic images ReNcell®CX cell viability after one and 7 days of cultivation (37°C, 5% CO 2 ) in five different GelNB hydrogel compositions . “high DTT:IKVAV” refers to a high crosslinker concentration, “low DTT:IKVAV” to a low crosslinker concentration. Encapsulated cell concentration 1 × 10 6 cells/mL. Green: Calcein-AM staining (viable cells), Red: Propidium iodide staining (dead cells).

    Article Snippet: The cells were cultivated with serum free ReNcell ® NSC Maintenance Medium (Merck KGaA) supplemented with 20 ng/mL epidermal growth factor (EGF; Thermo Fisher Scientific Inc.), 20 ng/mL basic fibroblast growth factor (bFGF; Bio-Techne) and 50 μg/mL gentamicin (Merck KGaA) in laminin-coated (20 μg/mL (Bio-Techne) in DMEM/F12) cell culture T-flasks.

    Techniques: Fluorescence, Concentration Assay, Staining

    Mean UnaG fluorescence of the ReNcell®CX-HRE-dUnaG hypoxia reporter cells in 2D cell culture after 24 h and 48 h cultivation in different oxygen concentrations (1%, 2%, 5%, 10%, 21%). Mean UnaG fluorescence determined via flow cytometry. Mean n = 3 ± SD; *** p < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization

    doi: 10.3389/fbioe.2025.1579580

    Figure Lengend Snippet: Mean UnaG fluorescence of the ReNcell®CX-HRE-dUnaG hypoxia reporter cells in 2D cell culture after 24 h and 48 h cultivation in different oxygen concentrations (1%, 2%, 5%, 10%, 21%). Mean UnaG fluorescence determined via flow cytometry. Mean n = 3 ± SD; *** p < 0.001.

    Article Snippet: The cells were cultivated with serum free ReNcell ® NSC Maintenance Medium (Merck KGaA) supplemented with 20 ng/mL epidermal growth factor (EGF; Thermo Fisher Scientific Inc.), 20 ng/mL basic fibroblast growth factor (bFGF; Bio-Techne) and 50 μg/mL gentamicin (Merck KGaA) in laminin-coated (20 μg/mL (Bio-Techne) in DMEM/F12) cell culture T-flasks.

    Techniques: Fluorescence, Cell Culture, Flow Cytometry

    Visualization of hypoxic response of ReNcell®CX-HRE-dUnaG hypoxia reporter cells using cell concentrations ranging from 1 × 10 6 to 15 × 10 6 cells/mL in GelNB hydrogel composition 2 (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV; 800 Pa) after 1-, 3- and 7-days incubation at 37°C and 21% O 2 .

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization

    doi: 10.3389/fbioe.2025.1579580

    Figure Lengend Snippet: Visualization of hypoxic response of ReNcell®CX-HRE-dUnaG hypoxia reporter cells using cell concentrations ranging from 1 × 10 6 to 15 × 10 6 cells/mL in GelNB hydrogel composition 2 (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV; 800 Pa) after 1-, 3- and 7-days incubation at 37°C and 21% O 2 .

    Article Snippet: The cells were cultivated with serum free ReNcell ® NSC Maintenance Medium (Merck KGaA) supplemented with 20 ng/mL epidermal growth factor (EGF; Thermo Fisher Scientific Inc.), 20 ng/mL basic fibroblast growth factor (bFGF; Bio-Techne) and 50 μg/mL gentamicin (Merck KGaA) in laminin-coated (20 μg/mL (Bio-Techne) in DMEM/F12) cell culture T-flasks.

    Techniques: Incubation

    Mean UnaG fluorescence intensity of ReNcell®CX-HRE-dUnaG reporter cells after 1-, 3- and 7-days cultivation in GelNB hydrogel (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV) using cell concentrations ranging from 1 x 10 6 to 15 x 10 6 cells/mL. Flow cytometric measurement after collagenase digestion of hydrogel. Mean n = 3 ± SD; ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: 3D culture of neural progenitor cells in gelatin norbornene (GelNB) hydrogels: mechanical tuning and hypoxia characterization

    doi: 10.3389/fbioe.2025.1579580

    Figure Lengend Snippet: Mean UnaG fluorescence intensity of ReNcell®CX-HRE-dUnaG reporter cells after 1-, 3- and 7-days cultivation in GelNB hydrogel (4.53% (w/v) GelNB, 2.59 mM DTT, 5.19 mM IKVAV) using cell concentrations ranging from 1 x 10 6 to 15 x 10 6 cells/mL. Flow cytometric measurement after collagenase digestion of hydrogel. Mean n = 3 ± SD; ** p < 0.01, *** p < 0.001.

    Article Snippet: The cells were cultivated with serum free ReNcell ® NSC Maintenance Medium (Merck KGaA) supplemented with 20 ng/mL epidermal growth factor (EGF; Thermo Fisher Scientific Inc.), 20 ng/mL basic fibroblast growth factor (bFGF; Bio-Techne) and 50 μg/mL gentamicin (Merck KGaA) in laminin-coated (20 μg/mL (Bio-Techne) in DMEM/F12) cell culture T-flasks.

    Techniques: Fluorescence